Agilent BioTek 405 LS Washer
The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.
Agilent BioTek 405 LS Washer
The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.
General
Microplate types | 96- and 384-well Low profile and standard height Solid and filter bottom (option) |
Onboard software | Create, edit or run multiple protocols |
Software (computer control) | LHC2 pc software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) |
Separation | Biomagnetic separation, vacuum filtration (optional) |
Shaking | Programmable up to 60 minutes Slow, medium, fast or variable |
Soaking | Programmable up to 60 minutes |
Ultrasonic Advantage™ | Yes (option) |
Verify™ clog detection | Yes (option) |
Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
Microplate types | 96- and 384-well Low profile and standard height Solid and filter bottom (option) |
Onboard software | Create, edit or run multiple protocols |
Software (computer control) | LHC2 pc software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) |
Separation | Biomagnetic separation, vacuum filtration (optional) |
Shaking | Programmable up to 60 minutes Slow, medium, fast or variable |
Soaking | Programmable up to 60 minutes |
Ultrasonic Advantage™ | Yes (option) |
Verify™ clog detection | Yes (option) |
Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
Washing
Manifold types | 96-well washing: 96-tube manifold - straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes |
Volume range | 25 to 3,000 µL/well, in 1 µL increments |
Wash cycles | 1 - 250 |
Buffer/reagent selection | Auto switching (internal) for up to 4 buffers (option) |
Supply bottle | 4 L or 10 L (optional) |
Dispense precision | <3% CV: 300 µL/well (96-well washing) <4% CV: 80 µL/well (384-well washing) |
Residual volume | <2 µL/well (96- & 384-well plates) 96-tube manifold for 96 wells; 192-tube for 384 wells |
Wash speed | 96-wells, 300 µL/well, 3 cycles; <30 seconds 384-wells,100 µL/well, 3 cycles: <80 seconds 384-wells, 400 µL/well, 1 cycle: <20 seconds |
Flow rates | High flow to low flow Optimized rates for cell assays |
Sterilization | Chemical |
Vacuum filtration | Selectable: Approximate final at 30 seconds: Lowest: -38 mmHg Low: -113 mmHg Medium: -200 mmHg High: -390 mmHg Highest: -506 mmHgVacuum filtration time range: 5 to 999 seconds |
Manifold types | 96-well washing: 96-tube manifold - straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes |
Volume range | 25 to 3,000 µL/well, in 1 µL increments |
Wash cycles | 1 - 250 |
Buffer/reagent selection | Auto switching (internal) for up to 4 buffers (option) |
Supply bottle | 4 L or 10 L (optional) |
Dispense precision | <3% CV: 300 µL/well (96-well washing) <4% CV: 80 µL/well (384-well washing) |
Residual volume | <2 µL/well (96- & 384-well plates) 96-tube manifold for 96 wells; 192-tube for 384 wells |
Wash speed | 96-wells, 300 µL/well, 3 cycles; <30 seconds 384-wells,100 µL/well, 3 cycles: <80 seconds 384-wells, 400 µL/well, 1 cycle: <20 seconds |
Flow rates | High flow to low flow Optimized rates for cell assays |
Sterilization | Chemical |
Vacuum filtration | Selectable: Approximate final at 30 seconds: Lowest: -38 mmHg Low: -113 mmHg Medium: -200 mmHg High: -390 mmHg Highest: -506 mmHgVacuum filtration time range: 5 to 999 seconds |
Physical Characteristics
Power | 100 - 240 Volts AC. 50/60 Hz |
Dimensions | 14" W x 17" D x 10" H (35.6 x 43.2 x 25.4 cm) |
Weight | With internal buffer switching - 36 lbs (16.5 kg) Without - 30 lbs (13.5 kg) |
Power | 100 - 240 Volts AC. 50/60 Hz |
Dimensions | 14" W x 17" D x 10" H (35.6 x 43.2 x 25.4 cm) |
Weight | With internal buffer switching - 36 lbs (16.5 kg) Without - 30 lbs (13.5 kg) |
Regulatory
Regulatory | CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. |
Configurations
Part #
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384-well
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96-well
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Optimized Cell Washing
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4-Buffer Switching
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Ultrasonic Advantage
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Verify Technology
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Biomagnetic Separation**
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Vacuum Filtration**
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405LSHT
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405LSHTV
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405LSU
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405LSUV
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405LSUS
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405LSUSQ
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405LSUVS
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405LSUVSQ
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405LSR
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405LSRS
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405LSRSQ
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405LSRVS
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405LSRVSQ
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* 96-well washing available with optional manifold PN 1170010, sold separately
** With available accessory module sold separately