Our Products

BioTek Cytation 1 Cell Imaging Multi-Mode Reader

Cytation™ 1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1’s multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.

BioTek Cytation 1 Cell Imaging Multi-Mode Reader

Cytation™ 1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1’s multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.


General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Image+ and Image Prime software available for full image analysis
Gen5 Secure available for 21 CFR Part 11
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) 0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
Models for both CO2 and O2 or CO2 only are available
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Image+ and Image Prime software available for full image analysis
Gen5 Secure available for 21 CFR Part 11
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) 0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
Models for both CO2 and O2 or CO2 only are available

Imaging System

Imaging mode Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, image stitching
Camera 16-bit gray scale, Sony CCD
Objective capacity 2 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software control
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutesLaser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features
Imaging mode Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, image stitching
Camera 16-bit gray scale, Sony CCD
Objective capacity 2 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software control
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutesLaser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features

Fluorescence Intensity

Light source Xenon flash
Detector PMT
Wavelength selection Deep blocking band pass filters / dichroic mirrors
Wavelength range Filters: 200 - 700 nm (850 nm option)
Dynamic range 7 decades
Sensitivity Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Light source Xenon flash
Detector PMT
Wavelength selection Deep blocking band pass filters / dichroic mirrors
Wavelength range Filters: 200 - 700 nm (850 nm option)
Dynamic range 7 decades
Sensitivity Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash)
100 amol (glow)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash)
100 amol (glow)

Fluorescence Polarization

Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 400 nm – 700 nm
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 400 nm – 700 nm
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein

Time-Resolved Fluorescence

Light source Xenon flash
Detector PMT
Wavelength selection Filters
Sensitivity Europium 40 fM (4 amol/well, 384-well plate)
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Sensitivity Europium 40 fM (4 amol/well, 384-well plate)

Absorbance

Light source Xenon flash
Detector photodiode
Wavelength selection Monochromator
Wavelength range 200 – 999 nm, 1 nm increment
Monochromator bandwidth 2.4 nm
Dynamic range 0 – 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Light source Xenon flash
Detector photodiode
Wavelength selection Monochromator
Wavelength range 200 – 999 nm, 1 nm increment
Monochromator bandwidth 2.4 nm
Dynamic range 0 – 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Reagent Injectors (option)

Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 – 200 µL
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 – 200 µL

Physical Characteristics

Power 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 65 lbs (29 Kg)
Power 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 65 lbs (29 Kg)

Regulatory

Regulatory CE and TUV marked. Models for In Vitro Diagnostic use are available.

Configurations

CYT1FAV
CYT1FA
CYT1V
Fluorescence and high contrast brightfield imaging
Monochromator absorbance
Filter/dichroic fluorescence
Luminescence / filtered luminescence