Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum applications reach. The multi-mode detection modules include filter- and monochromator-based fluorescence detection, luminescence and UV-Vis absorbance detection. Gen5™ software provides complete control over all imaging and data capture, plus powerful image and data analysis.
Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum applications reach. The multi-mode detection modules include filter- and monochromator-based fluorescence detection, luminescence and UV-Vis absorbance detection. Gen5™ software provides complete control over all imaging and data capture, plus powerful image and data analysis.
Enquiry about Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
General
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
| Temperature control | 4-Zone incubation to 65 °C with Condensation Control; +0.2 °C at 37 °C |
| Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes. |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Microplate Reader and Imager Software Gen5 Image+ and Image Prime software available for full image analysis Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2) Models for both CO2 and O2 or CO2 only are available |
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
| Temperature control | 4-Zone incubation to 65 °C with Condensation Control; +0.2 °C at 37 °C |
| Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes. |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Microplate Reader and Imager Software Gen5 Image+ and Image Prime software available for full image analysis Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2) Models for both CO2 and O2 or CO2 only are available |
Imaging System
| Imaging mode | Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast |
| Imaging method | Single color, multi-color, montage, time lapse, z-stacking |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | 16-bit Sony CMOS, standard or wide FOV |
| Objective capacity | 6 user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x |
| Phase objectives available | 4x, 10x, 20x, 40x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, auto LED intensity, auto exposure |
| Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software control Joystick control (option) |
| Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutesLaser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes 96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 5Oms integration time |
| Image Analysis Software option | Gen5 Image+: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features |
| Imaging mode | Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast |
| Imaging method | Single color, multi-color, montage, time lapse, z-stacking |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | 16-bit Sony CMOS, standard or wide FOV |
| Objective capacity | 6 user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x |
| Phase objectives available | 4x, 10x, 20x, 40x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, auto LED intensity, auto exposure |
| Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software control Joystick control (option) |
| Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutesLaser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes 96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 5Oms integration time |
| Image Analysis Software option | Gen5 Image+: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features |
Fluorescence Intensity
| Light source | Xenon flash |
| Detector | PMT for monochromator system PMT for filter system |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top) |
| Wavelength range | Monochromators: 250 - 700 nm (850 nm option) Filters: 200 - 700 nm (850 nm option) |
| Monochromator bandwidth | Variable, from 9 nm to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)Quad Monochromator: Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
| Light source | Xenon flash |
| Detector | PMT for monochromator system PMT for filter system |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top) |
| Wavelength range | Monochromators: 250 - 700 nm (850 nm option) Filters: 200 - 700 nm (850 nm option) |
| Monochromator bandwidth | Variable, from 9 nm to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)Quad Monochromator: Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
Fluorescence Polarization
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nM fluorescein |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nM fluorescein |
Time-Resolved Fluorescence
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
Absorbance
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increment |
| Monochromator bandwidth | 4 nm (230 - 285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increment |
| Monochromator bandwidth | 4 nm (230 - 285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
Alpha Detection
| Light source | 100 mW 680 nm laser |
| Detector | PMT |
| Wavelength selection | Filters (top) |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
| Light source | 100 mW 680 nm laser |
| Detector | PMT |
| Wavelength selection | Filters (top) |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
Reagent Injectors (option)
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates, Petri dishes |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 - 200 µL |
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates, Petri dishes |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 - 200 µL |
Physical Characteristics
| Power | 250 Watts maximum consumption. |
| Dimensions | 20" D x 16.5" W x 17.5" H (50.8 cm x 41.91 cm x 44.5 cm) |
| Weight | 80 lbs (36.3 kg) |
| Power | 250 Watts maximum consumption. |
| Dimensions | 20" D x 16.5" W x 17.5" H (50.8 cm x 41.91 cm x 44.5 cm) |
| Weight | 80 lbs (36.3 kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
Imaging Plus Multi-Mode Configurations
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CYT5FV
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CYT5FW |
CYT5FAV
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CYTFAW |
CYT5MV
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CYT5MW |
CYT5MPV
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CYT5MPW |
CYT5MFV
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CYT5MFW | CYT5MFAV | CYT5MFAW |
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Brightfield imaging
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Color brightfield imaging
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Phase contrast imaging
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Wide FOV camera
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FILTER-based
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Fluorescence intensity
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Fluorescence polarization
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Time resolved fluorescence
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Filtered luminescence
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Alpha detection
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Monochromator-based
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Fluorescence intensity
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Time resolved fluorescence
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Luminescence
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Take3 Plate compatible
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Imaging Only and Multi-Mode Only Configurations
| Part # |
CYT5V
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CYT5W |
CYT5PV
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CYT5PW | CYT5MF | CYT5MFA | CYT5F | CYT5FA | CYT5M |
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Phase contrast imaging
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Wide FOV camera
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FILTER-based
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Fluorescence intensity
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Fluorescence polarization
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Time resolved fluorescence
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Filtered luminescence
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Alpha detection
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MONOCHROMATOR-based
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Fluorescence intensity
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Luminescence
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OTHER
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Take3 Plate compatible
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All configurations include incubation to 65 °C and shaking, and are compatible with BioTek’s Dual Reagent Injection Module, CO2/O2 Gas Controller, and BioStack Microplate Stacker, and BioSpa Automated Incubator.
