BioTek Lionheart FX Automated Microscope

Lionheart™ FX Automated Microscope is a compact, inclusive microscopy system for a broad range of imaging workflows. It offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40°C and effective containment for CO2/O2 control, a humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays. Lionheart FX and Gen5 together comprise Augmented Microscopy™ – the automation of image capture, processing, analysis and development of publication-ready images and data.

BioTek Lionheart FX Automated Microscope

Lionheart™ FX Automated Microscope is a compact, inclusive microscopy system for a broad range of imaging workflows. It offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40°C and effective containment for CO2/O2 control, a humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays. Lionheart FX and Gen5 together comprise Augmented Microscopy™ – the automation of image capture, processing, analysis and development of publication-ready images and data.


General

Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control Incubation to 40 °C with optional environmental control cover
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Environmental control cover Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution Lead screw driven stage with 0.1 µm resolution
Humidity control Humidity chamber with rapid gas recharge (option)
Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control Incubation to 40 °C with optional environmental control cover
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Environmental control cover Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution Lead screw driven stage with 0.1 µm resolution
Humidity control Humidity chamber with rapid gas recharge (option)

Imaging System

Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit grayscale Sony CMOS
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Fluorescence:
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;
20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 100x, NA: 1.40
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit grayscale Sony CMOS
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Fluorescence:
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;
20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 100x, NA: 1.40
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller

Reagent Injectors (option)

Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume <1.65 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume <1.65 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Power 250 Watts maximum consumption.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight Without environmental control cover: 51 lbs (23.1 kg)
With environmental control cover: 58 lbs (26.3 kg)
Power 250 Watts maximum consumption.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight Without environmental control cover: 51 lbs (23.1 kg)
With environmental control cover: 58 lbs (26.3 kg)

Regulatory

Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.

Configurations

LFX

Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield, color brightfield and phase contrast imaging capabililty. Includes base Gen5 software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.