BioTek Lionheart LX Automated Microscope

BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.

BioTek Lionheart LX Automated Microscope

BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.


General

Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution
Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution

Imaging System

Imaging mode Fluorescence, high contrast brightfield and color brightfield
Imaging method Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
High NA: 20x, NA 0.75; 40x, NA 0.95
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
Imaging mode Fluorescence, high contrast brightfield and color brightfield
Imaging method Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
High NA: 20x, NA 0.75; 40x, NA 0.95
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller

Physical Characteristics

Power 60 Watts maximum consumption.
Dimensions 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
Weight 51 lbs (23.1 kg)
Power 60 Watts maximum consumption.
Dimensions 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
Weight 51 lbs (23.1 kg)

Regulatory

Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.

Configurations

LLX

Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.