Agilent BioTek Lionheart LX Automated Microscope
BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.
Agilent BioTek Lionheart LX Automated Microscope
BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.
General
Microplate types | 6- to 1536-well plates |
Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Support for labware up to 1.5" tall |
Software | Gen5 Microplate Reader and Imager Software included Gen5 Image and Image+ Software available for advanced image analysis (option) |
X/Y Stage resolution | Lead screw driven stage with 0.1 micron resolution |
Microplate types | 6- to 1536-well plates |
Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Support for labware up to 1.5" tall |
Software | Gen5 Microplate Reader and Imager Software included Gen5 Image and Image+ Software available for advanced image analysis (option) |
X/Y Stage resolution | Lead screw driven stage with 0.1 micron resolution |
Imaging System
Imaging mode | Fluorescence, high contrast brightfield and color brightfield |
Imaging method | Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode |
Light source | High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
Camera | 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size |
Camera binning | Optional 2x2 binning for focus and/or image capture |
Camera exposure range | 5 milliseconds to 4 seconds |
Image outputs available | Raw Images: 16-bit TIFF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF Movies: MP4, WMV |
Objective capacity | 6 onboard, user-replaceable objectives |
Objectives available | Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3 High NA: 20x, NA 0.75; 40x, NA 0.95 |
Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
Autofocus method | Image-based autofocus Laser autofocus option |
Image collection rate | Single well fastest frame rate capture: *Full Resolution: up to 10 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images |
Microscope stage control | Gen5 Software Control Optional joystick controller |
Imaging mode | Fluorescence, high contrast brightfield and color brightfield |
Imaging method | Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode |
Light source | High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
Camera | 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size |
Camera binning | Optional 2x2 binning for focus and/or image capture |
Camera exposure range | 5 milliseconds to 4 seconds |
Image outputs available | Raw Images: 16-bit TIFF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF Movies: MP4, WMV |
Objective capacity | 6 onboard, user-replaceable objectives |
Objectives available | Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3 High NA: 20x, NA 0.75; 40x, NA 0.95 |
Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
Autofocus method | Image-based autofocus Laser autofocus option |
Image collection rate | Single well fastest frame rate capture: *Full Resolution: up to 10 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images |
Microscope stage control | Gen5 Software Control Optional joystick controller |
Physical Characteristics
Power | 60 Watts maximum consumption. |
Dimensions | 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) |
Weight | 51 lbs (23.1 kg) |
Power | 60 Watts maximum consumption. |
Dimensions | 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) |
Weight | 51 lbs (23.1 kg) |
Regulatory
Regulatory | CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |
Configurations
LLX
Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.