Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
Synergy™ H1 is a configurable multi-mode microplate reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements.
Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
Synergy™ H1 is a configurable multi-mode microplate reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements.
Enquiry about Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
General
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported | Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control | 4-Zone™ incubation to 45 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2) Control Resolution: +0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2% at 1% O2 Models for both CO2 and O2 or CO2 only are available |
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported | Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control | 4-Zone™ incubation to 45 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2) Control Resolution: +0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2% at 1% O2 Models for both CO2 and O2 or CO2 only are available |
Absorbance
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | ±2 nm |
| Monochromator wavelength repeatability | ±0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | ±2 nm |
| Monochromator wavelength repeatability | ±0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
Fluorescence Intensity
| Light source | Xenon flash |
| Detector | PMT for monochromator system PMT for filter system |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top) |
| Wavelength range | Monochromators: 250 - 700 nm (850 nm option) Filters: 200 - 700 nm (850 nm option) |
| Monochromator bandwidth | Fixed, 16 nm |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)Quad Monochromator: Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
| Light source | Xenon flash |
| Detector | PMT for monochromator system PMT for filter system |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top) |
| Wavelength range | Monochromators: 250 - 700 nm (850 nm option) Filters: 200 - 700 nm (850 nm option) |
| Monochromator bandwidth | Fixed, 16 nm |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)Quad Monochromator: Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
Fluorescence Polarization
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nm fluorescein |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nm fluorescein |
Time-Resolved Fluorescence
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
Reagent Dispensers
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dispense accuracy | ±1 µL or 2% |
| Dispense precision | <2% at 50-200 µL |
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dispense accuracy | ±1 µL or 2% |
| Dispense precision | <2% at 50-200 µL |
Physical Characteristics
| Power | 130 Watts maximum consumption. |
| Dimensions | 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm) |
| Weight | 50 lbs (22.5 kg) |
| Power | 130 Watts maximum consumption. |
| Dimensions | 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm) |
| Weight | 50 lbs (22.5 kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
Configurations
|
Part #
|
H1M
|
H1F
|
H1MF
|
|---|---|---|---|
|
Monochromator fluorescence
|
•
|
•
|
|
|
Monochromator absorbance
|
•
|
•
|
|
|
Full-light luminescence
|
•
|
•
|
•
|
|
Filter/dichroic fluorescence
|
•
|
•
|
|
|
Fluorescence polarization
|
•
|
•
|
|
|
Time resolved fluorescence
|
• *
|
•
|
•
|
|
Filtered luminescence
|
•
|
•
|
|
|
Temperature control to 45°C
|
•
|
•
|
•
|
|
Gen5 data analysis software
|
•
|
•
|
•
|
|
Gas Controller Compatible Configurations**
|
H1MG | H1FG | H1MFG |
* Secondary mode
** These configurations have the same features as the non- Gas Controller Compatible configurations in the chart above. Gas Controller sold separately.
All configurations are compatible with the Dual Reagent Injector Module, sold separately.
