Agilent BioTek Cytation C10 Confocal Imaging Multi-Mode Reader
Cytation™ C10 Confocal Imaging Reader combines automated digital confocal and widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. The spinning disk confocal module provides exquisite resolution and optical sectioning capabilities in a wide variety of sample types. High quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality, at a significantly lower price than others. Cytation C10 also includes widefield fluorescence, brightfield and phase contrast optics. Its variable bandwidth monochromator-based multi-mode plate reading is based on the proven design of the market-leading BioTek Synergy™ products. Environmental controls enable live cell assays, and Gen5™ software is specifically designed to make sample detection and image capture quick and effortless. The new 3D viewer provides users with 3D reconstruction of their thicker samples captured with the confocal microscope. Cytation C10 brings affordable confocal to every laboratory.
Agilent BioTek Cytation C10 Confocal Imaging Multi-Mode Reader
Cytation™ C10 Confocal Imaging Reader combines automated digital confocal and widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. The spinning disk confocal module provides exquisite resolution and optical sectioning capabilities in a wide variety of sample types. High quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality, at a significantly lower price than others. Cytation C10 also includes widefield fluorescence, brightfield and phase contrast optics. Its variable bandwidth monochromator-based multi-mode plate reading is based on the proven design of the market-leading BioTek Synergy™ products. Environmental controls enable live cell assays, and Gen5™ software is specifically designed to make sample detection and image capture quick and effortless. The new 3D viewer provides users with 3D reconstruction of their thicker samples captured with the confocal microscope. Cytation C10 brings affordable confocal to every laboratory.
Enquiry about Agilent BioTek Cytation C10 Confocal Imaging Multi-Mode Reader
General
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | Monochromator: 6- to 384-well plates Imaging: 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates |
| Temperature control | 4-Zone incubation to 45 °C with Condensation Control |
| Shaking | Linear, orbital, double-orbital |
| Software | Gen5 Microplate Reader and Imager Software included Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Image+ and Image Prime software available for full image analysis (option) |
| Automation | BioStack and 3rd party automation compatible |
| CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller Models for both CO2 and O2 or CO2 only are available |
Imaging - Confocal Microscope
| Imaging modes | Fluorescence |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | Hamamatsu Orca sCMOS, 16-bit grayscale camera or Sony CMOS 16-bit grayscale camera |
| Objective capacity | 6-position automated turret for user-replaceable objectives |
| Objectives available | 20x, 40x, 60x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes |
| Imaging filter cubes available | CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield |
| Laser | 6-line |
| Automated functions | Autofocus, user-trained autofocus, autoexposure, auto-LED intensity |
| Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software Control Joystick controller (option) |
| Imaging methods | Single color, multi-color, time lapse, montage, z-stacking, z-stack montage |
| Field of view | Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification Sony CMOS: 0.70 mm + 5% at 20x magnification |
| Image collection rate | Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec |
Imaging - Widefield Microscope
| Imaging mode | Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield |
| Imaging method | Single color, multi-color, time lapse, montage, z-stacking, z-stack montage |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | Hamamatsu Orca sCMOS, 16-bit grayscale camera or Sony CMOS 16-bit grayscale camera |
| Objective capacity | 6-position automated turret for user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x |
| Phase objectives available | 4x, 10x, 20x, 40x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes, plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, autoexposure, auto-LED intensity |
| Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software Control Joystick controller (option) |
| Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes Laser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes |
| Image Analysis Software option | Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features |
Fluorescence Intensity
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (top/bottom) |
| Wavelength range | 250 – 700 nm (900 nm option) |
| Monochromator bandwidth | Variable, from 9 nm to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Reading speed (kinetic) | 96 wells, sweep mode: 10 seconds 384 wells, sweep mode: 20 second |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
Absorbance
| Light source | Xenon flash |
| Detector | Photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230 - 285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | + 2 nm |
| Monochromator wavelength repeatability | + 0.2 nm |
| OD accuracy | <1% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 10 seconds 384 wells: 20 seconds |
Reagent Injectors (Option)
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL, with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | < 2% at 50 - 200 µL |
Physical Characteristics
| Power | Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz. Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz. Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz. |
| Dimensions | 27" W x 18.5" H x 20" D (68.6 cm x 45.72 cm x 50.8 cm) |
| Weight | 122 lbs (53.3 Kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
Configurations
| Part # | C10PHC2 | C10MPHC2 | C10PWC | C10MPWC | C10PW | C10MPW |
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| Confocal 60 µm spinning disk |
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| Confocal 40 µm spinning disk |
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| Sony CMOS camera |
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| Multi-mode reading |
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| Phase contrast |
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